FastQCFastQC Report
Mon 27 Feb 2023
SRR3126673.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126673.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2532874
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216220.8536547810905715No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT43150.1703598362966338No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40290.15906831528137602No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG29790.11761343043514993No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC26180.10336084621659034TruSeq Adapter, Index 15 (95% over 23bp)
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT26040.10280811441864064No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG25920.10233434430611235No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG25570.10095251481123814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81000.086.395561
ACGGGAT7450.076.577824
ATAGCGG4150.075.046621
ATAGGGC15250.073.5835953
CGTAGGG6250.073.179832
AGTAGGG39650.073.136072
AGGGCAT21350.070.654265
ACGAGGG24500.069.476962
AGGGTAT11600.069.273845
TATAGGG20850.069.201432
GAATAGG18300.069.1065141
AGGGCAC15700.068.543625
AGAGGGC33500.068.1199653
TAGCGGG12350.067.95992
TAGAGGG42200.067.934562
GTAGGGT7850.067.870763
CGAAGGG23350.067.850342
AGTAAGG16800.067.6928941
AACGAGG10550.066.645231
AAGGGTA16500.066.5803154
Produced by FastQC (version 0.11.5)