FastQCFastQC Report
Mon 27 Feb 2023
SRR3126672.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126672.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530501
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206010.814107562099363No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40460.1598892867459843No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39320.1553842499963446No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG29030.11472036565091259No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC27130.10721197106817977TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG26280.10385295243906248No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG26270.10381343457283757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76750.085.401331
ATAGCGG5100.077.499871
TACGGGT1900.076.678953
CGTAGGG5950.075.075452
ACGGGAT5700.075.0299454
ATAGGGC16150.071.8774263
AGTAGGG41650.071.2372
TAAGGGA20300.070.610923
AGGGCAT21650.070.115285
GAATAGG18600.069.821311
CGAAGGG24600.069.766832
CGAGGGA15200.068.949223
GGTAAGG13700.068.347771
AAGGGCA32800.068.3459244
AACGAGG9500.067.8560941
AGAGGGC31900.067.4750063
TAGGGAC10200.067.26994
AGGGATG29100.066.37695
ACGAGGG23750.066.3242652
TATAGGG20850.066.30292
Produced by FastQC (version 0.11.5)