FastQCFastQC Report
Mon 27 Feb 2023
SRR3126671.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126671.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2687379
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217780.8103806720228148No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42550.1583327100494571No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41770.15543025379003111No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG29850.11107476838957213No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC29240.10880489875079026TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG28860.10739088159876223No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG28110.10460005827239106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84500.085.0451
ATAGCGG4800.074.514161
CGTAGGG7650.074.334782
AGTAGGG42550.073.670632
GTAGGGT8550.073.10613
GAATAGG17100.072.3812561
AGGGCAT20800.072.302745
TACGGGT1400.070.495173
TAAGGGA22950.070.034423
TAGAGGG47200.069.599052
AGGGTAC11200.069.236335
ATAGGGC16850.068.8914263
AGTAAGG16350.068.793371
AGAGGGC35100.068.68763
TAGCGGG12650.068.3589552
AAGGGCA36700.068.126124
ACGGGAT6700.068.0401154
TAGTAGG13100.067.8979951
AGGGATG29500.067.547915
AGAAGGG92150.067.218412
Produced by FastQC (version 0.11.5)