FastQCFastQC Report
Mon 27 Feb 2023
SRR3126670.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126670.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2443534
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214540.8779906479713397No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44410.18174496446540134No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40300.16492506345317887No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34370.14065693376887736No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76900.085.737251
CGTAGGG7700.079.004262
AGGGCAT22400.074.474845
AGGGATG36500.072.613185
AGTAGGG41600.072.5501252
CGAAGGG24950.071.445162
AGGGCGA11250.071.428715
GGTAAGG13750.070.707251
TAAGGGA22350.069.836773
GACCGAT1550.069.730918
TAGAGGG42200.069.2836462
ACTAGGG13200.068.9500962
ACGGGAT6300.068.862214
TATAGGG21150.068.673922
AGTAAGG16500.068.647821
ATAGCGG4450.067.87651
AAGGGAT44350.067.836084
AGAGGGC31550.067.557373
AGACGGG14450.067.554452
GTAAAGG19800.067.217661
Produced by FastQC (version 0.11.5)