FastQCFastQC Report
Mon 27 Feb 2023
SRR3126669.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126669.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448746
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203090.8293632741002945No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42870.17506919868373444No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39470.16118454098546767No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34720.14178685743641847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77750.084.755661
CGTAGGG7700.076.9585652
ATAGCGG5800.076.285321
AGGGATG36450.074.525665
ACGGGAT6250.072.940254
ATAGGGC15600.072.303973
AGGGTAC9500.070.743735
TGTAGGG19100.070.668462
AGTAAGG14650.070.3635251
AGGGCAT20250.070.32235
TAAGGGA20400.070.2663
AGGGCGA10000.070.026395
TAGCGGG14050.069.9595642
GTACGGG5850.069.94242
AGTAGGG40700.069.563142
TAGGGCA14600.069.208774
CGAAGGG25950.068.68772
GGTAAGG16000.068.54521
AGACGGG15400.068.407612
GAGGGAT27700.068.205874
Produced by FastQC (version 0.11.5)