FastQCFastQC Report
Mon 27 Feb 2023
SRR3126668.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126668.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2563002
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213540.8331636104848923No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44170.17233697047446705No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40530.15813487465089765No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36390.14198194148892587No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75700.083.980641
CGTAGGG7600.077.297442
ACGGGAT6500.074.471914
AGGGATG37050.073.8255
ATAGCGG5000.073.408161
AGGGCAT18150.073.2788245
ATAGGGC15650.071.771533
TCGTAGG1850.071.2206651
TAGGGTC6750.071.017454
AGACGGG16000.069.614072
AGGGTAC9950.069.432525
AGTAGGG41000.069.1197362
TGTAGGG19500.068.446682
TAGGGCA13200.068.359044
AGTAAGG15300.068.2780761
AAGGGTC16050.067.933134
TAGGGCG4500.067.884324
TATAGGG20950.067.52292
GAATAGG16600.067.466561
GAGGGAT28650.067.255514
Produced by FastQC (version 0.11.5)