FastQCFastQC Report
Mon 27 Feb 2023
SRR3126667.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126667.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521228
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71700.4713297414983158No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA57840.38021913874843216No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG36220.23809711627711294No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC34750.22843387053091319No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG34390.22606736136857852No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT32290.21226272458829315No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25550.16795641416013904No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG23420.15395456828299242No Hit
AAAGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT15540.10215431217411197No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA15310.10064237576484263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA1650.085.731194
CGCTACG4450.084.7679143
CGTAGGG4000.083.697842
CGTTTTT37950.083.676931
CGAAGGG13350.083.357872
TCCGCTA4600.082.060581
GCTACGA4650.081.121994
TAGGGCG1700.080.4360354
ATAGCGG2700.080.3889851
AGTACGG2150.079.0071641
AGGGCAT16000.079.006185
AGGGATG20150.078.1265265
CTACGAC4850.077.5134355
TAGGGCA8700.077.502974
AGAGGGC18700.077.1580663
GTAGCGG2650.076.564071
AGGGCGA6800.076.017325
TCGAGGG4750.075.445932
AGGGTAC5000.075.1880345
AGCGGGA8800.075.0147863