FastQCFastQC Report
Mon 27 Feb 2023
SRR3126665.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126665.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577113
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71050.4505067170202769No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA59160.37511579702912856No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG39920.25312073389795153No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG38030.2411368113762299No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC36770.23314752969508212No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT31490.19966863503122476No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24490.15528373680262605No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG22790.14450454723282352No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGTATTCA17690.11216697852341588No Hit
AAAGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT17150.10874300066006685No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA15950.10113416096373565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG2900.084.398521
CGCTACG4600.083.776873
CGTAGGG5050.083.756642
CGTTTTT36600.082.176811
AGTACGG1950.082.067991
GCTACGA4650.081.867964
CGAAGGG13300.081.272632
AGGGATG19500.081.2226645
AGGGCAT14000.076.875755
GGGCGAT6700.076.459886
ACGGGTA1600.076.372194
CTACGAC5050.075.383375
TAGCGGG8300.075.30812
TCCGCTA5200.075.1284261
AGGGAAT27500.074.513625
AATGCGG3100.074.398451
AAGGGCA18400.074.328794
TAAGGGA16250.074.327223
GGTAAGG8900.074.040191
GAAGGGC18250.073.907273
Produced by FastQC (version 0.11.5)