FastQCFastQC Report
Mon 27 Feb 2023
SRR3126664.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126664.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273797
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180510.7938703411078474No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36940.16245953354675022No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36620.16105219595240913No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG25340.1114435457518855No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22810.10031678289662621No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71900.083.766231
CGTAGGG6850.073.624492
AAGGGTA14600.073.282814
AGGGTAC11200.072.5881965
AGTAGGG38700.071.857182
ACGGGTA2250.071.223864
GTAGGGC10750.071.028993
TAGAGGG39750.070.551942
GGGCGAT11000.070.490276
CGAAGGG22750.069.612462
AGGGCAT22000.068.9950265
TAGCGGG11250.068.7100752
ATAAGGG33750.068.7100752
TAAGGGA18400.067.88243
ATAGGGC12300.067.826173
AAGGGCA33750.067.4531864
AGTAAGG13550.067.177671
TATAGGG17700.067.105232
GGGTACC11300.066.955366
AGGGATG25850.066.899865
Produced by FastQC (version 0.11.5)