FastQCFastQC Report
Mon 27 Feb 2023
SRR3126663.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126663.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284377
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174960.765898098256111No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36250.15868659157398274No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35280.15444035726152033No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG25930.11351016053829995No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23240.10173452105322371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65700.083.469231
ACGGGTA2400.078.328554
CGTAGGG7650.073.762972
AGGGTAC12700.072.160965
TAGGGCG4050.070.7858054
ATAGGGC12950.070.767883
AGTAAGG14400.070.2816241
AGTAGGG39450.070.088842
CGAAGGG22700.070.017962
AAGGGTA15700.069.148654
AGGGATG26400.069.071545
ATAGAGG15100.068.893961
TAGGGCA11400.068.022164
GAATAGG15300.067.993391
GTAGGGC10950.067.813223
TAGAGGG40300.067.560392
ATAGGGT9900.066.935313
ACGGGAT4850.066.86194
AGACGGG16650.066.652492
TGTAGGG15250.066.604332
Produced by FastQC (version 0.11.5)