FastQCFastQC Report
Mon 27 Feb 2023
SRR3126662.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126662.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2410844
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184950.7671587211781434No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36650.1520214497495483No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35530.1473757737954011No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26230.10880007167614329No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72300.084.611041
ACGGGTA2100.078.327084
TACGGGT2600.077.724563
CGTAGGG8050.074.143142
GTAGGGC9700.073.15913
AGGGCAT23300.072.813935
AGGGTAC12400.072.389385
AAGGGTA16200.071.364674
GAATAGG17600.070.585131
TAGGGCA13350.070.054334
ATAGAGG16950.069.682871
AGTAGGG36950.069.572252
TAACGGG7050.069.32782
AGTAAGG15500.069.218971
ACGGGAT4800.068.5361944
ATAGGGC14150.068.4185643
TAGCGGG13550.067.979812
CGAAGGG24850.067.8939742
AGGGATG25250.067.376795
ATAAGGG35300.067.099462
Produced by FastQC (version 0.11.5)