FastQCFastQC Report
Mon 27 Feb 2023
SRR3126661.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126661.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1820372
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323591.777603698584685No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35900.1972124378973089No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT27470.15090322197880432No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT24110.13244545620345732No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA23770.13057770609523767No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCT22570.1239856468897566No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT105100.087.351611
ATAGGGC13450.075.6916053
AGGGATG25600.073.2438055
GGTAAGG10400.071.1826251
TAAGGGA15600.070.698063
ATAGGGA15100.070.541993
TAGCGGG10850.070.372262
AAGGGTA11600.069.885444
TCGTAGG950.069.488371
AGAGGGC26350.069.222393
GTACGGG4500.069.126992
AGGGCAT15400.068.6593555
GAGGGAT20800.068.20554
AGTAGGG26300.068.0995252
ACGGGAT4300.067.9578254
AGGGAAT20900.067.904515
TAGGGCA10250.067.594244
AGAAGGG60800.067.054632
ATAGCGG4450.066.755681
GAATAGG10950.066.7461
Produced by FastQC (version 0.11.5)