FastQCFastQC Report
Mon 27 Feb 2023
SRR3126660.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126660.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1825174
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312631.7128777858987692No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35090.1922556424757311No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT27010.14798589066028772No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT23160.1268920113917906No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA23080.12645369701738024No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCT21660.1186736168715969No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG19020.10420924251605601No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102450.088.430111
ACGGGTA1600.079.3081744
ATAGGGC12600.074.226113
AGGGATG25600.073.617085
TAGGGCA9750.071.8217244
ACGGGAT4550.071.2708364
AGTACGG2350.070.094121
GGTAAGG11200.069.754371
TAGTAGG7900.069.1054461
ATAGCGG3900.068.784671
GTAAAGG13150.068.715841
AGTAGGG28750.068.696652
TGTAGGG12050.067.902592
GCATAGG4650.067.811481
AGAAGGG64700.067.665842
AGGGCAT15800.067.521645
TAGCGGG10800.067.488852
AGGGTAC8250.067.220585
TATAGGG16400.067.0959242
AGAGGGC25450.066.8490143
Produced by FastQC (version 0.11.5)