FastQCFastQC Report
Mon 27 Feb 2023
SRR3126658.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126658.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321643
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66330.5018753173133743No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA50750.3839917436100369No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50250.38020857372225325No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG34410.2603577516772684No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG34000.2572555523692858No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC33650.25460733344783726No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT30610.2316056605301129No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24270.18363506635301666No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA13910.10524778627814017No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCGTCTGTATTCGT13370.10116196279933386No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG1950.091.915384
AATGCGG2550.086.9750751
AGGGATG25400.086.954125
GTACGGG2700.085.5995562
ACGGGTC1450.084.57554
CGCTACG9650.084.069863
AGTAAGG7150.083.157541
GCTACGA9800.082.783074
CGAGGGA6050.082.6398853
TCCGCTA9900.082.46091
AAGGGTC4600.082.029744
GGGTACG1500.081.453196
AGGGATT13350.081.312395
AGGGCGA5050.080.95695
ATTGCGG1750.080.894751
AGTACGG1400.080.894751
TAACGGG3750.080.498532
GGATGGC16700.080.196317
AGGGCAT12550.080.130175
CTACGAC10150.080.0951165