FastQCFastQC Report
Mon 27 Feb 2023
SRR3126656.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126656.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366137
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67010.4905071746098671No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA52790.386418053240634No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50030.36621510141369423No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC38340.28064535255248924No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG37720.27610700830151No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG37200.2723006550587533No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT30630.22420884581853795No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG25010.18307095115643598No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTCTGTAT20440.1496189620806698No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA14740.10789547461198988No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTT14570.1066510898980117No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCGTCTGTATTCGT14490.10606549709143373No Hit
AGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT14170.10372312586512188No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTA156.905423E-493.9899758
ACGGGTA1250.086.470784
TAGGGCG2400.086.157484
AGGGATG23950.085.356245
TTAGCGG850.083.029571
AGGGCAT12500.082.335215
GGTACGC401.036824E-982.241237
AGGGCGT1550.081.8622365
ATAGCGG2300.081.8262561
TAGCGGG6050.081.558562
AGGGCGA5600.081.402035
TACGGGA1850.081.285653
CGTTTTT37900.081.189341
ACGGGAT5200.080.433724
TAGAGGG18100.079.4471742
AGTACGG1600.079.397031
GGTAAGG8300.079.36161
CGGGATA2550.079.246455
GGGCGAT6050.079.231226
CGCTACG9450.079.068033
Produced by FastQC (version 0.11.5)