FastQCFastQC Report
Mon 27 Feb 2023
SRR3126654.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126654.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896419
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354261.868047092968379No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38910.2051761767837171No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT31020.16357144702726562No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT26100.13762781326278634No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT25090.13230198600625706No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA24060.1268706968238559No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC19730.10403818987259673No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG19130.1008743320964407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT123800.088.929661
AGGGATG23900.074.133725
GGTAAGG11100.072.936091
TAGGGCA11050.072.303454
AGTAGGG32200.072.147012
GTACGGG4650.071.804672
ATAGGGC12100.071.466883
GCATAGG4100.071.177851
ACGGGAT4650.070.748544
AGGGAAT20450.070.093695
CGTAGGG5650.069.9162452
GTAGGGC10100.069.3325653
TGTAGGG14200.069.21582
GTAGGGT7150.069.016943
ACGGGTA2000.068.1464
ATGTAGG6000.067.465891
TAGGGTA4950.067.410194
TAAGGGA14800.066.685283
GGGCGAT7950.066.209956
GAGGGAT19250.065.918214