FastQCFastQC Report
Mon 27 Feb 2023
SRR3126652.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126652.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2232930
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177740.795994500499344No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39680.1777037345550465No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23760.10640727653800164No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69250.083.917771
GGTAAGG13850.074.9265751
GAATAGG14200.070.7545241
AGTAGGG35550.070.413262
TAGACGG3700.070.11711
ATAGCGG3800.069.513221
CGTAGGG7000.069.364722
TAGGGCG3900.068.896954
AGGGATG30250.068.819935
ATAGGGA18700.068.819393
ATAGGGC12850.068.23383
ACGGGAT5750.068.04564
AGTAAGG15600.068.03321
GGGCGAT10400.067.778796
TAAGGGA17700.067.647263
AGAAGGG78450.066.760662
AGGGTAC9300.066.7001655
GTAAGGG36900.066.687382
GTAGGGC8950.066.364723
TATAGGG14100.066.1981052
Produced by FastQC (version 0.11.5)