FastQCFastQC Report
Mon 27 Feb 2023
SRR3126651.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126651.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2242689
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167450.7466483315341539No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40150.17902616011404168No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22830.101797440483277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62600.082.788581
GAATAGG14650.071.65141
ACGGGTA2100.071.614364
CGTAGGG5650.071.5702442
AGTAGGG36200.071.309312
ACGGGAT4950.071.207464
AGTAAGG15050.070.998111
ATAGGGC13900.070.664443
GGTAAGG14600.070.6071551
ATAGCGG4100.070.0331041
AGGGATG29550.069.6604165
TAGGGCA12050.067.862784
TAGGGCG4050.067.304244
GTAGGGC9150.067.285223
CGAAGGG22750.067.171412
CGAGGGA11850.067.025153
TAAGAGG15950.066.991941
AGGGTAC9450.066.641145
GTAAGGG39300.066.4022752
GTACGGG5600.066.331772
Produced by FastQC (version 0.11.5)