FastQCFastQC Report
Mon 27 Feb 2023
SRR3126650.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126650.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2359729
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179050.7587735710329448No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41130.17429967593736398No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23720.10052001734097434No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69450.081.990351
GAATAGG15150.072.686351
AGTAGGG37850.072.6341862
ATAGGGC12100.072.628533
AATGCGG5500.071.017441
GGTAAGG13150.070.858031
ATAGCGG5600.070.589621
AGGGATG29950.068.884065
ATAGAGG16600.068.3216861
AGGGCAT19450.067.653375
AGAAGGG81650.066.9382862
AGACGGG15950.066.883072
GTAGGGC10150.066.6726153
ATAGGGA19350.066.54583
CGAAGGG23000.066.405882
ACGGGAT5050.066.072094
TAGCGGG12200.065.484752
ATAAGGG33850.065.2514652
AAGAGGG80000.065.2054752
GGGGGGA310000.065.0955666
Produced by FastQC (version 0.11.5)