FastQCFastQC Report
Mon 27 Feb 2023
SRR3126649.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126649.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100564
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211051.0047301581860872No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31600.15043578772177377No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA30040.14300921085955962No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG23060.10978004002734504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76400.086.804081
ACGGGTA2500.079.2007454
ATAGCGG4750.077.4549561
TAGGGCA11000.072.8578344
CGTAGGG5250.072.7371142
AGGGATG29800.072.697115
CGAAGGG19100.072.07422
AGGGCAT16700.071.192895
ACGGGAT5300.071.159694
AGGGTAG7700.070.184255
AGAGGGC27650.069.564073
GTACGGG4950.069.526122
AGTAGGG36150.069.119082
AAGGGCA30400.069.009114
AGTACGG2600.068.9379651
GTAGGGT7950.068.787713
TAAGAGG16800.068.786781
GAATAGG12550.068.778951
GGTAAGG10300.068.233431
GTAGGGC9000.068.095883
Produced by FastQC (version 0.11.5)