FastQCFastQC Report
Mon 27 Feb 2023
SRR3126648.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126648.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112605
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199650.9450417849053656No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32190.15237112474882905No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA28420.1345258578863536No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG22410.10607756774219507No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78100.086.681881
ACGGGTA2050.080.236134
CGTAGGG6100.073.226022
CGAAGGG20400.072.833062
AGGGATG30150.072.6364065
AAGGGTA13100.072.1075064
AGGGCAT17100.071.455065
TAGGGCA10600.070.936524
GAATAGG13950.070.821261
CGAGGGA12700.069.93813
AGGGTAC9250.068.079895
TAAGAGG16550.067.6546251
AAGAGGG75800.067.612812
AGTAAGG14250.067.349421
AGTAGGG35200.067.188812
AGAGGGC27100.066.5913243
GAGGGTA11200.066.297144
AAGGGCA30850.066.2658544
ATAGCGG5400.066.212251
ATAGGGA19600.066.177253
Produced by FastQC (version 0.11.5)