FastQCFastQC Report
Mon 27 Feb 2023
SRR3126647.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126647.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292425
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213880.9329858119676762No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31780.13863048954709534No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA30840.13453002824519886No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24560.107135456994231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84750.086.644131
AGGGATG33250.074.346845
ACGGGTA3050.073.9622044
AGGGTAC11250.071.017415
CGAAGGG23100.070.5969542
AGGGCAT18450.070.304185
GAATAGG14300.069.410051
TATAGGG19200.068.78182
TAGGGCA11700.068.687654
AGTAGGG41950.068.674732
AAGGGTA15450.068.441964
GAGGGAT26400.067.468914
CGTAGGG6050.066.80542
ATAGGGC12950.066.77543
GGTAAGG15200.066.538161
ATAGCGG5100.066.410731
GTAGGGC11750.066.39553
GTAAGGG36550.066.091282
TAAGGGA18650.066.022343
AAGAGGG80700.065.807192
Produced by FastQC (version 0.11.5)