FastQCFastQC Report
Mon 27 Feb 2023
SRR3126646.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126646.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976479
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203631.0302664485683886No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27080.13701132164824417No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24980.12638636686754579No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG22360.11313047090305538No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20100.10169599575811329No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75300.086.799691
TAGGGTA6250.072.3859944
AGGGATG30750.071.9815
TAGGGCA9800.071.651214
CGAAGGG18300.069.7881242
CGTAGGG5000.069.7469252
AGTAGGG35300.069.688192
AGGGCAT17100.069.2542655
AGAGGGC29100.068.989013
TAGAGGG36500.068.817312
AGGGTAC9300.068.722365
ATAGCGG4200.068.490521
TAAGGGA16500.067.976123
TATAGGG17350.067.362092
ACGGGAT4200.067.323294
TAGGGCG4550.067.323294
GTAGGGC9550.067.11183
AAGAGGG69100.066.972542
ACGGGTA1900.066.9689564
TAGGGAC9150.066.95544
Produced by FastQC (version 0.11.5)