FastQCFastQC Report
Mon 27 Feb 2023
SRR3126645.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126645.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981074
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189880.9584700016253811No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26540.13396773669231943No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25140.12690086286529428No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21340.10771934819194035No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20300.10246967049186452No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72550.085.303731
AGGGATG29050.074.096165
TACGGGT1700.069.114233
AAGGGTA13550.069.022424
AGTAGGG35000.068.7856142
AGAGGGC26550.068.6821063
CGAAGGG19600.068.372982
CGTAGGG5300.068.3141252
GTACGGG5100.068.227152
GGTAAGG11900.068.023841
AGGGCAT18150.067.065565
ATAGGGC13600.067.04083
AGAAGGG69200.066.998722
AGGGTAT11300.066.9612965
AAGGGCA27650.066.799594
AGTAAGG12800.066.550041
ATAGAGG14150.065.854951
GAGGGAT24300.065.564674
TAGGGTA6100.065.488564
ATAGGGT8900.065.479913
Produced by FastQC (version 0.11.5)