FastQCFastQC Report
Mon 27 Feb 2023
SRR3126644.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126644.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2089993
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200620.9599075212213629No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27060.12947411785589713No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26810.12827794160076136No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21740.10401948714660768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69400.086.704721
AGGGATG28500.073.543355
ATAGCGG4550.071.345951
TAGGGCA11150.071.230384
ATAGGGC13350.070.052763
TAAGAGG15950.069.9067841
GAATAGG12800.069.835341
CGAAGGG18950.069.6868062
AGGGCAT18800.068.992945
AGAGGGC27750.068.9262853
AGTAGGG36350.068.6504752
GTACGGG4700.067.993042
TATAGGG18600.067.460842
GGTAAGG12450.067.263941
GTAGGGC9450.067.135993
AGTAAGG15000.066.493051
AAGGGCA30600.066.3461464
AGATAGG14000.066.2018051
AAGAGGG75500.066.042252
AGAAGGG73100.066.024712
Produced by FastQC (version 0.11.5)