FastQCFastQC Report
Mon 27 Feb 2023
SRR3126642.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126642.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1573215
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82530.5245945404792097No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC58230.37013377065436065No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA46300.29430179600372486No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT27780.1765810776022349No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG27090.1721951545084429No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG20200.12839948767333137No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC19310.12274228252336776No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG17250.10964807734480031No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC16460.10462651322292249No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT37600.083.853491
AGGGATG43900.083.290765
GGGCGAT5650.082.3509146
TAGGGCG1600.082.246944
CGAAGGG14450.081.366162
CGAGGGA8200.080.240923
GGATGGC29350.079.905047
AGGGCAT14650.078.597765
AAGGGCA19450.078.5317844
GAGGGAT28100.078.4419254
CGCTACG10350.077.649293
AGGGTGC5950.077.408885
TACGGGT850.077.408883
AATGCGG2800.077.30971
AGTAAGG11400.076.778971
AGGGCGA6000.076.763825
GATGGCA28300.076.0607768
ATAGCGG2850.075.953391
GGAATGC18550.075.754597
GGGATGG37150.075.273116