FastQCFastQC Report
Mon 27 Feb 2023
SRR3126641.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126641.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644880
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86490.5258134331987744No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC62460.37972374884490057No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA47240.2871942026166043No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT29190.17745975390302027No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG27130.16493604396673314No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG21920.1332620008754438No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC18750.11399007830358446No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG18670.11350372063615582No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC17450.10608676620786928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT41000.084.934071
AGGGATG46200.083.207815
GACCGAT1250.082.711418
ACGGGAT6500.080.2532044
CGGGATA3700.080.0187155
AGTACGG1900.079.2556841
GGATGGC31450.079.047447
TAGGGCG2500.078.95184
AGGGCAT14350.078.5981145
CGCTACG10600.078.472983
CGAGGGA8550.078.050383
GGATGAC31450.078.001447
CGTAGGG3800.077.9129642
GGAATGC19400.077.759977
AATGCGG3450.077.74811
GAGGGAT31700.077.386294
TAGCGGG7650.076.789412
GGGCGAT7300.076.608496
GGGATGA37950.076.034276
CGAAGGG14350.075.978172