FastQCFastQC Report
Mon 27 Feb 2023
SRR3126640.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126640.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9409573
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3458023.6750020431320314No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT443570.4714029000040703No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT213900.22732168611689393No Hit
CGTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT192350.20441947790829615No Hit
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT190150.2020814334508059No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCT188700.20054044960382367No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT150780.16024106513653702No Hit
GAATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT127740.13575536318172993No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG111160.11813500995209879No Hit
CGTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC100460.1067636119088507No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTC96410.10245948461210727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT971450.090.6166151
AGGGACT233000.071.339715
ATAGGGC48650.071.1558763
GTTTTTT1284850.068.908072
AGTAGGG138400.068.81072
TAGGGCA37400.067.751194
GACTTAA196350.067.4948048
GGTAAGG54250.066.9775541
AGATAGG44800.066.7990651
GGACTTA201700.066.403527
GTAGGGT29600.065.078553
ATAGGGA56900.065.060383
AATAGGG111650.064.7531052
ATAGCGG18150.064.65431
AAGGGGC288550.064.4736564
GGATAGG50000.064.3762051
GTAGGGA61050.064.340833
GTAGGGC41700.064.040283
GATAGGG119250.063.5094832
CAGGGAC214000.063.2527284