FastQCFastQC Report
Mon 27 Feb 2023
SRR3126639.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126639.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6428707
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2061683.2069901459189225No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT262190.4078425101657301No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC207260.3223976454363218No Hit
CGTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT195500.3041046978809269No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCT187830.2921738383783862No Hit
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT181650.282560707775296No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT124370.1934603645803114No Hit
GAATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT114640.17832512821007396No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG107280.16687648076043907No Hit
CGTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC95910.14919018707805473No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTC94030.14626580430559363No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT87230.13568824959669185No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC82370.12812840902532968TruSeq Adapter, Index 21 (95% over 22bp)
GGGCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT71830.11173319922653185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT601850.090.5712051
ATAGGGC33950.068.938193
AGGGACT149200.068.8879855
GTTTTTT797250.068.676022
GTAGGGA43850.068.485963
GGTAAGG32900.068.183611
AGTAGGG92250.067.452072
AGATAGG32600.067.3684841
GACTTAA123550.066.870248
ATAGAGG37900.066.63351
AATAGGG70750.065.96242
TAGGGAC30500.065.9493944
ATAGGGA37850.065.5599063
TAGGGCA22250.065.056044
GGACTTA129250.064.86767
GGATAGG32800.064.376891
GATAGGG83600.064.1435552
ATGTAGG17100.063.804221
TATAGGG40300.063.6738662
AGTAAGG36300.063.6110231
Produced by FastQC (version 0.11.5)