FastQCFastQC Report
Mon 27 Feb 2023
SRR3126636.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126636.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10878653
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3731243.429873165363396No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT583200.5360957831819804No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT289230.26586931304822387No Hit
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT221710.20380280536570106No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT198630.18258694343867757No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT168830.1551938461498864No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG166740.15327265241386043No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT158740.14591880079270841No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT118060.10852446529915055No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC117610.10811081114546074No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA108950.10015026676556373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1102950.090.2228551
ATAGGGC56050.071.830923
AGGGACT301950.070.551535
AGTAGGG163950.068.616042
TAGGGCA46200.067.8817754
GGTAAGG59550.067.810681
GACTTAA260500.067.760038
GTTTTTT1481500.067.746092
AGATAGG55050.067.533421
TAGGGAC51200.066.770964
GGACTTA267900.066.361997
AATAGGG130650.065.99332
GTAGGGA76850.065.930953
ATAGGGA68100.065.6205753
TAGTAGG39000.065.242141
GGATAGG60500.064.4093861
AGTAAGG63400.064.138761
ATAGAGG70600.064.07161
GATAGGG147650.064.008092
TATAGGG75450.063.846532