FastQCFastQC Report
Mon 27 Feb 2023
SRR3126635.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126635.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7547836
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2279213.019686702254792No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT355410.47087668571495195No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC263480.3490801866919207No Hit
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT223290.29583313680901385No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT185430.245673064438602No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG175550.2325832198791813No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT172380.22838334060252502No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT166440.22051353527024167No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC122000.16163573241389984No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT118870.1574888484593465No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT114350.15150037706171676No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC98610.13064671781421855No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTC89110.11806032881477552No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG75810.10043938421555529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT690600.090.270131
ATAGGGC36800.071.3909153
AGGGACT195100.070.965265
AGTAGGG109550.069.4141542
GACTTAA166200.068.486028
GGATAGG40400.067.748511
GTTTTTT927450.067.70652
GGTAAGG38150.067.67621
GGACTTA172000.066.806817
AGATAGG37800.066.685461
TAGGGCA27800.065.423914
ATAGAGG48200.065.078411
CAGGGAC177550.065.0096744
GATAGGG99050.064.293442
TAGTAGG26050.064.268841
AAGGGGC234850.064.216924
ATAGGGA40700.064.20363
GTAGGGA49150.064.066393
TAGGGAC34050.064.042934
ACTTAAT177500.063.887789
Produced by FastQC (version 0.11.5)