FastQCFastQC Report
Mon 27 Feb 2023
SRR3126634.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126634.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11002079
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3494623.176326946934302No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT589320.5356442177882925No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT291710.2651407974801854No Hit
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT233020.21179633412921323No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT201990.1835925737308376No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC182980.1663140211954486No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG173950.15810648151135798No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT171380.15577055936428016No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT159520.1449907785610338No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC122310.11116989798019083No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT120240.10928843539480129No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA110260.10021742254350291No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1046000.090.1390841
AGGGACT310100.072.215475
ATAGGGC57300.072.09473
AGTAGGG162250.069.1162342
GACTTAA268950.068.865868
TAGGGAC50100.068.759914
GTAGGGA74650.067.803953
GGACTTA276550.067.636097
GTTTTTT1415150.067.378362
TAGGGCA45300.067.227334
GGTAAGG63200.067.0807951
AGATAGG55150.066.12211
ATAGGGA64200.065.663873
ATAGAGG71450.065.196371
CAGGGAC286300.064.922354
ACTTAAT285050.064.613519
AAGGGGC348950.064.566014
GATAGGG150500.063.7963182
AATAGGG136700.063.5981942
TATAGGG73100.063.5500562