FastQCFastQC Report
Mon 27 Feb 2023
SRR3126633.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126633.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13222593
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4329263.274138438655716No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT722020.5460502338686519No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT360720.272805795353453No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT248880.1882232932678182No Hit
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT233420.1765311841633483No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT184880.13982128921309156No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG181430.13721211868201647No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT171280.12953586335146217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1281800.089.963941
ATAGGGC67850.072.824493
AGGGACT382450.070.401255
AGTAGGG197800.069.312592
TAGGGCA54250.068.80984
GTTTTTT1736900.067.055922
GACTTAA330700.066.689428
GTAGGGA93900.066.239773
ATAGAGG86850.065.76761
TAGGGAC66200.065.62114
GGTAAGG73250.065.260321
GGACTTA342000.065.104287
AGATAGG69400.064.541741
AAGGGGC427300.064.519454
GATAGGG178200.064.034342
ATAGGGA83500.063.7922633
AATAGGG164600.063.6408922
CAGGGAC348150.063.3473634
GTAGGGT40450.062.99493
ATAGCGG29450.062.9468651