FastQCFastQC Report
Mon 27 Feb 2023
SRR3126632.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126632.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561998
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133000.8514735614258149No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC56210.3598596156973312No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG24340.15582606379777694No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22020.1409732918992214No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC21860.13994896280276925Illumina Single End Adapter 1 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCAAATGC21200.13572360527990432No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG19600.12548031431538323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT47450.083.829681
ATAGCGG3300.078.455981
AGGGATG28200.075.159045
TAGGGTA5300.073.5963064
TCCGCTA10350.073.225581
ACGGGAT4600.072.536124
CTACGAC10500.072.059365
TAGGGCA8000.071.6677254
CGCTACG10600.071.379553
TAGGGCG3350.070.1421364
AGGGTAC7900.069.600535
CGGGATG6550.069.595995
AGGGAAT18150.069.392415
GGATGGC25400.069.382727
GAGGGAT20500.069.2319954
GCTACGA11000.069.211154
CGTAGGG4350.069.184492
AAGGGAT31200.069.1372154
GAATAGG8900.068.759171
GAGGGTA8550.068.7064744
Produced by FastQC (version 0.11.5)