FastQCFastQC Report
Mon 27 Feb 2023
SRR3126631.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126631.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591597
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132360.8316175514279054No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC57650.36221480688892976No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG26060.16373491530833495No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC23150.1454513925321548Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG21880.13747198568481847No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21120.1326969075714518No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCAAATGC20250.12723069973115053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT43750.082.564421
AGTACGG1700.080.234081
AGGGATG27500.079.975035
TCCGCTA12150.074.324971
ACGGGTA1650.074.050954
TAGACGG1800.073.163641
ATAGCGG2900.072.9834441
TAGGGCA8250.072.9117054
CTACGAC12250.072.888465
ACGGGAT4000.072.840514
TAGCGGG8050.071.8043062
TACGACC12450.071.717576
AGGGAAT18150.071.461765
GAGGGAT21800.071.353094
AAGGGAT29050.070.854794
AGGGTAC7250.070.6528555
AGTAAGG9250.070.16931
AAGAGGG48700.070.1530762
AGAGGGC19900.069.900433
TAGGGTA5650.069.8674
Produced by FastQC (version 0.11.5)