FastQCFastQC Report
Mon 27 Feb 2023
SRR3126630.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126630.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781885
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168570.9460206466747292No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC65490.3675321359122502No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG28560.1602797037968219No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC25350.14226507322301943Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24230.13597959464275192No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG24190.1357551132648852No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55050.084.403361
AGGGATG31900.078.227455
ACGGGTA1450.077.7854844
TAGACGG2350.076.045911
TAGGGTA6550.074.6186454
CTACGAC14050.074.590565
CGTAGGG5250.074.2974852
TCCGCTA14250.074.2553561
AGGGAAT21950.072.794695
AGGGTAC7650.072.489635
AGGGCAT14150.072.40285
CGCTACG14650.071.535663
TACGACC14600.071.4587556
AAGGGAT30850.070.988194
ACGACCA14450.070.899637
AAGAGGG57750.070.798262
ATAGGGC10400.069.589343
GGTAAGG9750.069.457311
CGAAGGG17550.069.3550342
GAGGGAT25150.069.138364
Produced by FastQC (version 0.11.5)