FastQCFastQC Report
Mon 27 Feb 2023
SRR3126629.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126629.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737829
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409281.494907096096944No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC35750.13057791410639596Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT34730.12685233445916455No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31980.11680787952790331No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG31290.11428763447242321TruSeq Adapter, Index 23 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT137100.087.302041
TAGGGCA14900.079.169164
ATAGGGC17300.075.520923
GTAGGGC13450.074.775633
AGTACGG3350.071.625891
ATAGCGG5550.071.2083741
AGTAGGG44500.070.893372
GTACGGG7000.070.523582
TAGGGTA8500.070.2187964
AGGGAAT26850.068.963695
AGGGATG39200.068.337235
AGGGTAC13500.068.232445
AGGGCAT23400.067.884315
GTAGGGA20650.067.821093
TAGGGCG5450.067.261524
TGTAGGG19650.067.233682
AAGAGGG85150.067.141652
GGTAAGG17550.067.020491
ATAGGGA22750.066.9317553
TAGAGGG44650.066.548572
Produced by FastQC (version 0.11.5)