FastQCFastQC Report
Mon 27 Feb 2023
SRR3126628.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126628.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799154
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT413521.4773034995573662No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC38660.13811315847573946Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT35940.12839593677232478No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG34770.12421610243666478TruSeq Adapter, Index 23 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32340.11553490804721712No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG29230.10442440823191579TruSeq Adapter, Index 23 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT141600.087.916091
ATAGCGG5950.075.8808061
CGTAGGG8850.075.3990252
ATAGGGC17300.074.970683
TAGGGCA15400.072.014464
GAATAGG17450.071.690871
ATAGGGA23500.071.5885543
ACGGGAT6600.070.488734
AGTAGGG47850.069.922792
CCGTACA7700.068.9633
GTAGGGC13700.068.945183
TAGGGAT17200.068.8494644
GTACGGG6500.068.68012
GTAGGGT10750.068.630893
AGGGAAT27650.068.321815
AGGGATG37350.068.31845
TAGGGTA9650.067.688664
GTAGGGA21950.067.652063
AGTACGG3700.067.3677141
AGTAAGG18500.066.8592761
Produced by FastQC (version 0.11.5)