FastQCFastQC Report
Mon 27 Feb 2023
SRR3126627.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126627.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3126886
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT524041.6759165508432352No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC39980.1278588346361204Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT39170.1252683980164291No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG37980.12146269483441353TruSeq Adapter, Index 23 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36780.11762501095338941No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT163800.087.430871
TAGGGCA17550.075.522894
CGTAGGG8800.075.308332
GTAGGGC14450.072.5343253
ATAGGGC21200.072.053343
AGTAGGG55600.071.853962
GGTAAGG20700.070.434071
ATAGCGG7150.070.3832241
GAATAGG20050.069.1988751
GTAGGGA23450.069.1485063
TAGAGGG52750.069.053522
ATAGGGA27950.068.945933
TAGGGTA11900.068.724074
AGGGCAT26000.068.693695
AGGGATG42800.068.634555
ACGGGTA3450.068.117314
TAGGGCG4850.067.836414
TAGGGAT21400.067.646224
AATAGGG46950.067.272912
ATGAGGG49550.067.252622
Produced by FastQC (version 0.11.5)