FastQCFastQC Report
Mon 27 Feb 2023
SRR3126626.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126626.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954253
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164800.8432889702612711No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG30560.15637688671835223No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC29040.1485989787402143No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26970.1380066961647238No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTACAACGT25610.13104751534217934No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG24470.1252140843585759No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22250.11385424507471653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59350.082.941691
AGGGTAC9200.075.602365
CGTAGGG6150.074.9340442
ACGGGAT4750.074.204314
GTACGAT452.6357156E-973.104988
GGTAAGG11150.072.174391
AGTAGGG27700.069.603692
TAAGGGA15850.069.3821953
ATAGAGG14050.069.335541
GTAGGGA16100.067.721033
AAGGGTA15350.067.662084
ATAGGGA17500.067.405783
CGAAGGG19150.067.283722
AGGGCAT14950.067.271955
TAGGGCA10150.066.674224
GTAGGGC7700.066.5268863
AGAGGGC26550.066.2016143
TACGGGT1350.066.14263
TAGGGAC9200.065.3858264
AGTAAGG10800.064.9268651
Produced by FastQC (version 0.11.5)