FastQCFastQC Report
Mon 27 Feb 2023
SRR3126625.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126625.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027675
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166940.8233074827080278No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG35290.17404169800387143No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC33080.16314251544256353No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG28140.13877963677611058No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26720.13177654209870912No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTACAACGT23950.1181155757209612No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22440.1106686229302033No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55550.082.971171
TAGCGGG8650.073.341422
ACGGGAT5400.072.229744
CGTAGGG5800.072.1096952
AGGGTAC8550.071.4510965
AGGGCAT16300.070.92175
TAGGGCA9950.069.898894
AGATAGG11900.069.558611
AGTAAGG11350.069.199961
AGTAGGG29550.068.700192
GGTAAGG12400.068.6502461
CGAAGGG20700.068.559592
ATAGGGA17200.068.303543
AGAGGGC28700.068.278793
ATAGGGT7350.067.132623
AGTTAGG5150.066.665451
ATAGCGG5300.066.553461
ATAGAGG12750.066.396861
AATAGGG27150.066.291922
ATAGGGC11700.065.87033
Produced by FastQC (version 0.11.5)