FastQCFastQC Report
Mon 27 Feb 2023
SRR3126624.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126624.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259028
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216900.9601474616516484No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG38160.1689222090208709No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC37620.16653180040265106No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG32450.14364585122450896No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30220.13377434896778614No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26300.1164217530725604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72500.083.481361
ACGGGAT6350.075.491254
GTAGGGT9100.072.819533
AGGGTAC11050.072.7283865
GGTAAGG14300.071.034071
AAGGGTA15200.070.495514
AGATAGG13650.069.937871
TAAGGGA20450.069.863523
TAGGGCA10600.069.608774
CGTAGGG6350.069.5703662
ATAGGGA19900.069.432763
AGTAGGG33550.068.6394352
ATAGGGC12750.068.191733
ATAGAGG15750.067.480281
TATAGGG18400.067.1750642
TACGGGT2100.067.138573
AGTAAGG12850.066.60661
TAAGGGT9350.066.34873
GAATAGG11700.066.320391
CTACGAC6800.065.657585
Produced by FastQC (version 0.11.5)