FastQCFastQC Report
Mon 27 Feb 2023
SRR3126623.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126623.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242389
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCACCTTTC44661.8424928523984176No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG20280.8366716311383767TruSeq Adapter, Index 27 (95% over 24bp)
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATG19030.7851016341500646No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC16790.6926881995470091TruSeq Adapter, Index 27 (100% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15140.6246158035224371No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA13990.5771714062931899No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG11660.481044931906976TruSeq Adapter, Index 27 (95% over 24bp)
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCCACCTTTCTC11360.4686681326297811No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT9140.3770798179785386TruSeq Adapter, Index 27 (100% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG8580.3539764593277748No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG8560.35315133937596177No Hit
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT8050.3321107806047304TruSeq Adapter, Index 27 (95% over 21bp)
ATCCGTACACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC7930.32716006089385247No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGT7210.2974557426285846No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCCACCTTTCTCG6720.27724030380916626No Hit
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG6500.26816398433922334TruSeq Adapter, Index 27 (100% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG6160.2541369451584024No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC5730.23639686619442302No Hit
ATCCGTACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG5170.21329350754365917No Hit
GATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC5030.20751766788096818TruSeq Adapter, Index 27 (95% over 22bp)
AAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT4730.19514086860377328No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4520.18647710910973683No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG4430.18276406932657835No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG4410.18193894937476535No Hit
GACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC4320.17822590959160686TruSeq Adapter, Index 27 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATG4170.17203750995300943No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC4120.16997471007347695No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT3860.159248150699908No Hit
ATCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC3670.15140951115768456TruSeq Adapter, Index 27 (100% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT3610.14893415130224555No Hit
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT3320.13696991200095712TruSeq Adapter, Index 27 (100% over 22bp)
ACACCGAGATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGC3200.13201919229007916No Hit
ACACCGAGATCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATG3080.1270684725792012No Hit
AATGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT3040.12541823267557523No Hit
GACACACCCACACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTA2970.12253031284422972No Hit
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT2940.12129263291651023TruSeq Adapter, Index 27 (100% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGG2880.11881727306107125No Hit
GACACACCCACACACCGAGATCCTGTCTCTTATACACATCTGACGCCACC2720.1122163134465673No Hit
ACACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT2580.10644047378387633TruSeq Adapter, Index 27 (95% over 21bp)
GGAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT2510.10355255395253085TruSeq Adapter, Index 27 (95% over 21bp)
GACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT2480.10231487402481136TruSeq Adapter, Index 27 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGG201.8280867E-594.071031
ATCGAGG301.3145836E-894.071031
CGTAGGG353.5652192E-1094.0321962
CGGGTAT201.835598E-593.99345
AGGGTAC750.093.99345
TAGGTTG156.897512E-493.993394
GCGGGTA301.3220415E-893.993394
TAGGGTA301.3220415E-893.993394
GATTAGA156.897512E-493.993399
CGTTTTT19750.089.7842561
TAGTAGG650.086.83481
AAGGGTA1150.085.820054
TATAAGG500.084.663931
CGGGATT500.084.594065
ATGACGG452.7284841E-1183.618691
GCGGGAT452.7284841E-1183.549694
TGACGGG850.082.969582
AACGAGG401.024091E-982.312161
TACGGGA401.031367E-982.2442253
TAGGGTG401.031367E-982.2442254
Produced by FastQC (version 0.11.5)