FastQCFastQC Report
Mon 27 Feb 2023
SRR3126622.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126622.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246677
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCACCTTTC39681.608581262136316No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG20910.8476671923203217TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC18840.7637517887764161TruSeq Adapter, Index 27 (100% over 24bp)
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATG16810.6814579389241803No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16090.6522699724741261No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA13390.5428150982864232No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG13110.5314642224447355TruSeq Adapter, Index 27 (95% over 24bp)
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCCACCTTTCTC11350.46011586001126986No Hit
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT8760.3551202584756585TruSeq Adapter, Index 27 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT8690.3522825395152365TruSeq Adapter, Index 27 (100% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG7770.31498680460683404No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG7650.310122143531825No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCCACCTTTCTCG7280.2951227718838806No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC7100.287825780271367No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC6740.27323179704633993No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGT6610.2679617475484135No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG6390.2590432022442303No Hit
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG6070.2460707727108729TruSeq Adapter, Index 27 (100% over 23bp)
GATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC5660.22944984737125876TruSeq Adapter, Index 27 (95% over 22bp)
AAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT5260.21323431045456204No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4760.19296488930869113No Hit
ATCCGTACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG4660.18891100507951694No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC4510.18283017873575566No Hit
ATCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC4480.18161401346700343TruSeq Adapter, Index 27 (100% over 21bp)
GACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC4350.17634396396907698TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG4300.17431702185448988No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG4220.17107391447115053No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATG3790.15364221228570155No Hit
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT3750.15202065859403188TruSeq Adapter, Index 27 (100% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT3750.15202065859403188No Hit
GACACACCCACACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTA3630.14715599751902286No Hit
GAATGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT3610.146345220673188No Hit
ACACCGAGATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGC3500.14188594802109641No Hit
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT3170.12850813006482162TruSeq Adapter, Index 27 (100% over 22bp)
AATGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT3170.12850813006482162No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGG3140.12729196479606936No Hit
ACACCGAGATCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATG3040.12323808056689517No Hit
ACACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT3030.12283269214397774TruSeq Adapter, Index 27 (95% over 21bp)
GACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT2940.119184196337721TruSeq Adapter, Index 27 (100% over 22bp)
GGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT2760.11188720472520745TruSeq Adapter, Index 27 (95% over 23bp)
GGAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT2570.1041848246897765TruSeq Adapter, Index 27 (95% over 21bp)
GACACACCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG2530.10256327099810683No Hit
GACACACCCACACACCGAGATCCTGTCTCTTATACACATCTGACGCCACC2510.101752494152272No Hit
AGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT2490.10094171730643717TruSeq Adapter, Index 27 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG156.882674E-494.044821
GATACTA156.893781E-494.006698
ACGGGAT156.893781E-494.006694
TACGACC254.907579E-794.006696
TAGGGCG254.907579E-794.006694
GATAGTC156.893781E-494.006698
AGCGGGT201.8343744E-594.006683
TAGGGTC201.8343744E-594.006684
TACGAGG750.087.775171
CGTTTTT20500.087.622251
ATAGGGT500.084.606023
TGTAGGG1450.084.264782
AGTATGG401.027729E-982.289221
AGGGTAT401.031367E-982.255855
AGAGGGC800.082.255853
AGGGAAT6800.082.255855
TAGCGGG800.082.239182
AGGGCAT1150.081.744945
ATAGCGG353.839341E-880.6098561
AATGCGG353.839341E-880.6098561
Produced by FastQC (version 0.11.5)