FastQCFastQC Report
Mon 27 Feb 2023
SRR3126621.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126621.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262121
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG24450.9327753213210693TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21260.8110758008705903No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC20810.7939081569198958TruSeq Adapter, Index 27 (100% over 24bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCACCTTTC17470.666486088485852No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG13670.5215148729022093TruSeq Adapter, Index 27 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT10070.38417372129665306TruSeq Adapter, Index 27 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT9110.3475494142018381TruSeq Adapter, Index 27 (100% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG9010.34373438221279484No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG8970.3422083694171775No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCCACCTTTCTCG7900.301387527134415No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG7420.2830753735870076No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA7240.27620831600672974No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC7150.2727747872165908No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATG5950.22699440334807205No Hit
GATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC5640.21516780418203807TruSeq Adapter, Index 27 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA5460.20830074660176026No Hit
AAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT5140.19609264423682193No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG4990.1903700962532571No Hit
GACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC4970.18960708985544844TruSeq Adapter, Index 27 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATG4530.17282094910365822No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT4530.17282094910365822No Hit
GAATGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT4500.17167643950694525No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC4470.1705319299102323No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG4440.16938742031351933No Hit
AATGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT4150.15832382754529395No Hit
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG4090.15603480835186803TruSeq Adapter, Index 27 (100% over 23bp)
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCCACCTTTCTC3950.1506937635672075No Hit
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT3910.14916775077159022TruSeq Adapter, Index 27 (100% over 22bp)
GACACACCCACACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTA3720.14191918999240807No Hit
ATCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC3590.1369596484066519TruSeq Adapter, Index 27 (100% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGG3420.1304740940252784No Hit
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT3300.1258960556384265TruSeq Adapter, Index 27 (100% over 22bp)
ACACCGAGATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGC3260.12437004284280924No Hit
GACACACCCACACACCGAGATCCTGTCTCTTATACACATCTGACGCCACC2920.11139893408006227No Hit
AGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT2850.10872841168773201TruSeq Adapter, Index 27 (95% over 21bp)
ACACCGAGATCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATG2840.10834690848882768No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGG2750.10491337969868877No Hit
GACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT2700.10300586370416714TruSeq Adapter, Index 27 (100% over 22bp)
GGAACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT2680.10224285730635853TruSeq Adapter, Index 27 (95% over 21bp)
ATCCGTACACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC2670.1018613541074542No Hit
GGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT2650.10109834770964554TruSeq Adapter, Index 27 (95% over 23bp)
GACACACCCACACACCGAGATCCGTACACCCTGTCTCTTATACACATCTG2650.10109834770964554No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACGG156.880708E-494.053251
TATAAGG409.094947E-1294.053251
AGTACGG156.880708E-494.053251
ACGGGAT254.9107075E-793.9994354
GCACCGA156.8963855E-493.999438
CAGGGCG156.8963855E-493.999434
TAGGGCG301.3220415E-893.999434
CGGGACG156.8963855E-493.999435
GGTTAGC301.3220415E-893.999437
GGTACCG201.8352948E-593.999427
GGGTACG201.8352948E-593.999426
GGCCGAT201.8352948E-593.999427
GTTAGCC201.8352948E-593.999428
AACGGGT409.094947E-1293.999423
GTACAGG1000.089.35061
TAGCGGG950.089.052092
CGTTTTT22150.087.683961
CGAGGGC600.086.1661453
AGGGTAG550.085.4540255
GAGTAGG1000.084.6479341
Produced by FastQC (version 0.11.5)