FastQCFastQC Report
Mon 27 Feb 2023
SRR3126620.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126620.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510237
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGACCTTA52591.030697499397339No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44840.8788072993530457No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG25570.5011396664687194TruSeq Adapter, Index 23 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22800.4468511691625657No Hit
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC22540.44175549793527324TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG19750.3870750259193277TruSeq Adapter, Index 23 (95% over 21bp)
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATG18760.3676722777846373No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA14830.2906492473105635No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGGACCTTATC11520.22577743284003318No Hit
CGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT11430.2240135466459704TruSeq Adapter, Index 20 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT9850.19304754457242418No Hit
ACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG8250.16168956778908625TruSeq Adapter, Index 20 (95% over 22bp)
GACACACCCACACACCTGTCTCTTATACACATCTGACGCGGACCTTATCG8030.15737784598137727No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC7450.1460105793974173No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCGGACCTTATCGT7400.14503064262293797No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG7240.14189484494460416No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG7100.1391510219760621No Hit
AGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT6330.12405999564908071TruSeq Adapter, Index 20 (95% over 21bp)
GATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC6060.11876833706689245No Hit
ATCCGTACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG5650.1107328555161621No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC5500.10779304519272417No Hit
AAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT5430.10642113370845313No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCC5270.10328533603011934No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG5170.10132546248116071No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA800.093.998435
CGTAGGG950.089.077332
CGTTTTT28550.088.51411
GTACGGG1000.084.6234742
ACGGGTC452.7284841E-1183.554164
CGCTACG3950.083.289753
GCTACGA3950.082.09994
TCCGCTA4150.081.644831
TACGGGT353.860805E-880.570083
GCGGGAT2050.080.242574
TACGACC4050.080.072756
CGAGGGA3000.079.8986743
AGGGAAT10600.079.809995
CGGGTAG301.4551206E-678.332025
CGGCATA301.4551206E-678.332029
TACGGGA900.078.332023
CTACGAC4150.078.143285
CGACCAA4100.077.949928
AGCGGGA2850.077.507483
GCACGGG850.077.4332352
Produced by FastQC (version 0.11.5)