FastQCFastQC Report
Mon 27 Feb 2023
SRR3126619.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126619.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519840
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGACCTTA48650.9358648815020008No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45370.8727685441674361No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG26290.5057325330871036TruSeq Adapter, Index 23 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24460.47052939365958757No Hit
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC24150.46456602031394273TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG21710.4176285010772545TruSeq Adapter, Index 23 (95% over 21bp)
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATG17180.33048630347799324No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA13890.26719759926131115No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGGACCTTATC11870.22833948907356108No Hit
CGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT11380.21891351184979993TruSeq Adapter, Index 20 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT9700.1865958756540474No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCGGACCTTATCG8700.16735918744228995No Hit
ACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG7940.15273930440135425TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG7120.13696522006771314No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG6890.13254078177900894No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCGGACCTTATCGT6820.1311942136041859No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC6570.12638504155124652No Hit
AGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT6270.1206140350877193TruSeq Adapter, Index 20 (95% over 21bp)
GATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC6260.12042166820560173No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCC6120.11772853185595566No Hit
GGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT5650.10868728839642967No Hit
AAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT5300.10195444752231456No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC5270.10137734687596182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGA800.088.112283
CGTTTTT27400.086.686641
GTAGCGG600.086.228881
GTACGGG1150.085.81372
GTCGAGG1400.083.989171
CGCTACG4400.082.238143
CGTAGGG1200.082.238132
AAGGGAC5000.081.7681964
CGAAGGG4750.081.125142
GCTACGA4500.080.410624
TCGAGGG2400.080.2800752
AGAGGGC4650.078.827343
TACGACC4600.078.662566
AGGGATG9600.078.322025
TCCGCTA4700.078.056321
AGGGAAC3800.077.909815
AGGGAAT10600.077.5831455
AAGGGTC1700.077.40064
AGGGTAT2550.077.400595
AGGGATT7600.077.291485
Produced by FastQC (version 0.11.5)