FastQCFastQC Report
Mon 27 Feb 2023
SRR3126618.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126618.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571563
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59921.0483533748685623No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG31140.5448218306643362TruSeq Adapter, Index 23 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27600.4828864009741708No Hit
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC26500.463640928471577TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG24240.4241002304207935TruSeq Adapter, Index 23 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGACCTTA20870.36513910102648356No Hit
CGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT12360.21624912739278082TruSeq Adapter, Index 20 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT11930.208725897232676No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCGGACCTTATCG9350.16358651627204698No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG8440.14766526174717398No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG7940.13891731970054044No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA7850.13734269013214642No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCC7470.13069425417670494No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG7260.12702011851711884No Hit
AGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT6970.12194631213007141TruSeq Adapter, Index 20 (95% over 21bp)
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC6390.11179869935597651No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATG6190.1082995225373231No Hit
GATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC6020.10532522224146769No Hit
AAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT5900.10322571615027565No Hit
GGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT5890.10305075730934299No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTA301.3254976E-893.992664
CGTTTTT33750.085.8220441
CGCTACG4400.085.447873
CGAAGGG4700.084.9933552
TCCGCTA4450.084.532151
CGGGATA452.7284841E-1183.549035
GCTACGA4500.083.549034
GTAGCGG850.082.978251
ACGGGAT1600.082.2435764
CGTAGGG1200.082.2435762
ATAGCGG750.081.503081
CTACGAC4650.080.85395
TACGACC4650.080.85396
GTAGGGC2400.080.28543
CCGCTAC4750.080.1411062
TAGCGGG2700.080.067812
TAAGGGA5950.078.985423
TGAAGGG8700.078.86742
AGGGAAT10750.078.691525
TACGGGA900.078.327213
Produced by FastQC (version 0.11.5)