FastQCFastQC Report
Mon 27 Feb 2023
SRR3126617.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126617.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869356
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153860.823064199649505No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35400.18937002903673777No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTAGCGGT32310.17284027226488696No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29100.15566858319121665No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG25180.1346987946651146No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC24890.13314745826905094No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG23460.1254977650057025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56000.082.193641
TAGGGTA6900.077.645344
CGTAGGG4350.076.751062
TAGGGCA7900.074.955444
AGGGTAT9650.074.024585
AGGGATG32350.072.636585
GTAGGGA16250.072.590543
AAGGGTA11050.071.025434
AGTAGGG32200.070.827382
AGAGGGC26800.070.669163
AGTAAGG8200.070.021821
GTAGGGT8550.069.8067253
AGGGCAT13650.069.5475
AGGGTAC9200.069.472155
CCGTACA16100.068.888363
TAAGGGA16650.068.870823
CGTACAC16250.068.8308644
TATAGGG12750.068.5990452
GAGGGAT28700.068.2831954
ACGGGTA1800.067.882924
Produced by FastQC (version 0.11.5)