FastQCFastQC Report
Mon 27 Feb 2023
SRR3126616.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126616.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953567
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156600.8016105923165163No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37560.19226368995790777No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29310.15003324687609895No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTAGCGGT29150.14921423222239116No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG28010.143378752814723No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC27380.14015388261574852No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG25840.13227086657381087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56350.082.493751
ACGGGTA1800.078.3200454
CGTAGGG4900.076.721672
ATAGCGG4200.073.935451
AGTAGGG33000.072.766442
AGGGATG34250.072.031585
TAGGGCA8750.071.9649354
GTAGGGC9600.070.977553
TAGGGTA6850.070.659544
GGTAAGG11950.070.476311
TAAGGGA19400.070.245823
CCGTACA14450.070.244143
ACGGGAT5900.070.0898064
AGGGAAT24500.070.008535
AGGGCAT17150.069.871535
GTAGGGA17500.069.54823
AAGGGTA12850.069.116684
TAGGGAT15100.069.0876164
AGGGTAC8850.069.027835
TGTAGGG15700.068.24322
Produced by FastQC (version 0.11.5)